The disclosure that international monitors were not physically present is likely to feed critics of a nuclear deal between Iran and world powers, who have poured scorn on measures used to check if Tehran's atomic programme is peaceful.
In a mark of the high stakes at play it drew a quick reaction from the UN's International Atomic Energy Agency, whose chief insisted that "the integrity of the sampling process and the authenticity of the samples" was not compromised.
The samples were taken under "established procedures", IAEA director general Yukiya Amano said, noting "significant progress" is being made in its long-running probe of whether Iran ever sought to develop a nuclear bomb.
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